E-FAS, vol. 18, no. 4, pp.367-372, December, 2015
DOI. http://dx.doi.org/10.5657/FAS.2015.0367

Effects of Dietary Supplementation of Two types of propolis
on growth performance, feed utilization, innate immunity
and disease resistance of olive flounder Paralichthys olivaceus

G. L. B. E. Gunathilaka1, Yong-Kap Hur2, Se-Jin Lim3 and Kyeong-Jun Lee 1*

1Department of Marine Life Sciences, Jeju National University, Jeju 63243, Korea
2Unique Biotech Co. Ltd, 105 B1 Center, Youngdong University, Yeongdong 29131, Korea
3NeoEnBiz (Neo Environmental Business Co.), Dangjin 31723, Korea

We investigated the effects of dietary supplementation of two types (powder or liquid) of propolis on innate immunity and disease
resistance of olive flounder Paralichthys olivaceus against Edwerdsiella tarda. A total of 600 fish averaging 30 g were randomly
distributed into 24 tanks in groups of 25. Three tanks were assigned to each of eight experimental diets: 0 (Control), 0.25, 0.5,
0.75 and 1 % propolis in powder form and 0.25, 0.5 and 1 % propolis in liquid form (PP0.25, PP0.5, PP0.75, PP1, LP0.25, LP0.5
and LP1, respectively). Fish were fed each experimental diet twice daily for four weeks to apparent satiation. At the end of the
feeding trial, lysozyme and myeloperoxidase activities and total immunoglobulin level were significantly higher in fish fed the
PP1 and LP0.5 diets compared to those fed the control diet. The PP1 diet was also associated with a significant increase in antiprotease
activity compared to the control diet. After challenge with E. tarda, fish fed the LP0.5 diet showed numerically higher
survival compared to the other groups. This study indicates that non-specific immune responses of olive flounder can be enhanced
by dietary supplementation with powder and liquid forms of propolis, and that the optimal level would be 1% in powder form or
0.5% in liquid form. It seemed that growth performance and feed utilization are not affected by the propolis supplementation in
diets for olive flounder.

Keyword : Paralichthys olivaceus, Propolis, Innate immunity, Olive flounder, Edwerdsiella tarda

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