Table 4
Purification of β-secretase inhibitory peptide from sea hare muscle hydrolysate obtained by trypsin treatment

Purification step

IC50 value (μg/mL)

Purification fold*

Trypsin hydrolysate

340.31 ± 0.10

1.00

Sephadex gel filtration (F2)

101.11 ± 0.03

3.37

RP-HPLC (C)

89.43 ± 0.03

3.81

Purified peptide (C-1)

60.12 ± 0.05

5.66