Table 1
Dietary formulation and proximate composition of the six experimental diets for L. vannamei (% dry matter). All three experimental (trial 1, 2, and 3) diets were produced using the same diet formulation

Ingredients

Experimental diets

P25

P30

P35

P40

P45

P50

Brown fish meal1

18.0

18.0

18.0

18.0

18.0

18.0

Gelatin2

0.50

1.50

2.50

3.50

4.50

5.50

Casein3

2.00

6.00

10.0

14.0

18.0

22.0

Soybean meal

4.00

4.00

4.00

4.00

4.00

4.00

Squid liver meal

5.00

5.00

5.00

5.00

5.00

5.00

Wheat flour

34.5

34.5

34.5

34.5

34.5

34.5

Starch

30.0

25.0

20.0

15.0

10.0

5.00

Fish oil

2.00

2.00

2.00

2.00

2.00

2.00

Vitamin/mineral premix4

2.00

2.00

2.00

2.00

2.00

2.00

Choline chloride

1.00

1.00

1.00

1.00

1.00

1.00

Lecithin

1.00

1.00

1.00

1.00

1.00

1.00

Chemical composition (% dry matter)

 Dry matter

89.0

88.8

88.4

88.9

89.0

88.6

 Crude protein

25.9

30.4

35.8

40.2

45.0

50.1

 Crude lipid

8.12

8.09

8.18

8.17

8.27

8.23

 Crude ash

5.19

5.13

5.15

5.28

5.14

5.33