Table 5
Purification of antioxidant peptide from black eelpout muscle hydrolysate by pepsin treatment

Purification step

EC50 value (μg/mL)

Purification folda

Pepsin hydrolysate

830.01 ± 0.05

1.00

Sephadex gel filtration (B)

650.32 ± 0.14

1.28

RP−HPLC (F1)

87.45 ± 0.05

9.49

Purified peptide (F1–1)

56.12 ± 0.01

16.24