Primer

Sequence (5’-3’)

MCH Forward

CTGTTGTCGTCAGCTCTCCG

MCH Reverse

GGGATGCAGATCTACCCACTG

POMC Forward

GAACATCCGAGCTGTCAGGA

POMC Reverse

CATGGAGTAGGAGCGCTTGG

MC1R Forward

GTTGGCCATCTTCAAGAACCG

MC1R Reverse

TCACGTCGATGACGTTGTCC

ASIP Forward

GGCCCACCTCCTGTTGTTATT

ASIP Reverse

GACAGTTCTGAAGAGCCGACA

β-actin Forward

GAGGGGTATCCTGACCCTGA

β-actin Reverse

CTCCTCAGGGGCAACTCTC