Table 1. LC-MS/MS operating conditions for the analysis of PSTs and TTX

	PSTs				TTX
Spray voltage	5.0 kV				3.5 kV
Capillary temp.	350°C				320°C
Sheath gas	17 (Arb)				50 (Arb)
Aux gas	19 (Arb)				10 (Arb)
Polarity	Positive				Positive
Detection	Selected reaction monitoring (SRM)				SRM

Mass spectrometer	Toxins	Parent ion (m/z)	Fragment ion (m/z)	Collision energy (eV)	Parent ion (m/z)	Fragment ion (m/z)	Collision energy (eV)
	STX	300	282	20	320	302	35
	NEO	316	298	20		256	40
	GTX1	412	332	20		162	40
	GTX2	396	316	20
	GTX3	396	298	20
	GTX4	412	314	20
	GTX5	380	300	20
	dcSTX	257	196	20
	dcGTX2	353	273	20
	dcGTX3	353	335	20
	C1	396	316	20
	C2	396	298	20

LC-MS/MS, liquid chromatograph-mass spectrometer; PST, paralytic shellfish toxins; TTX, tetrodotoxin; STX, saxitoxin; NEO, neosaxitoxin; GTX, gonyautoxin; dcSTX, decarbamoylsaxitoxin; dcGTX, decarbamoylgonyautoxin; C, protogonuautoxin.