Table 1. LC-MS/MS operating conditions for the analysis of PSTs and TTX

PSTs TTX
Spray voltage 5.0 kV 3.5 kV
Capillary temp. 350°C 320°C
Sheath gas 17 (Arb) 50 (Arb)
Aux gas 19 (Arb) 10 (Arb)
Polarity Positive Positive
Detection Selected reaction monitoring (SRM) SRM
Mass spectrometer Toxins Parent ion (m/z) Fragment ion (m/z) Collision energy (eV) Parent ion (m/z) Fragment ion (m/z) Collision energy (eV)
STX 300 282 20 320 302 35
NEO 316 298 20 256 40
GTX1 412 332 20 162 40
GTX2 396 316 20
GTX3 396 298 20
GTX4 412 314 20
GTX5 380 300 20
dcSTX 257 196 20
dcGTX2 353 273 20
dcGTX3 353 335 20
C1 396 316 20
C2 396 298 20
LC-MS/MS, liquid chromatograph-mass spectrometer; PST, paralytic shellfish toxins; TTX, tetrodotoxin; STX, saxitoxin; NEO, neosaxitoxin; GTX, gonyautoxin; dcSTX, decarbamoylsaxitoxin; dcGTX, decarbamoylgonyautoxin; C, protogonuautoxin.