Table 3. Results of the comet assay performed at various concentrations of ethylene glycol (EG) as the cryoprotective agent (CPA) with ASP (artificial seminal plasma of small yellow croaker) as the diluent in large yellow croaker (Larimichthys crocea)

Parameters	Control	EG concentration (%)
		5%	10%	15%	20%
Head length (µm)	60.6 ± 0.65a	54.82 ± 0.1c	56.04 ± 0.76b	55.96 ± 0.74b	52.10 ± 0.68d
Tail length (µm)	34.23 ± 0.52e	51.39 ± 0.91b	40.79 ± 1.08d	50.12 ± 1.42c	54.97 ± 1.53a
Head intensity (%)	93.02 ± 0.69a	82.95 ± 0.65c	86.71 ± 0.94b	81.44 ± 0.97d	75.99 ± 1.12e
Tail intensity (%)	6.98 ± 0.69e	17.05 ± 0.65c	13.29 ± 0.94d	18.56 ± 0.97b	24.01 ± 1.12a
Tail DNA (%)	2.19 ± 0.22e	6.77 ± 0.29c	4.66 ± 0.36d	7.42 ± 0.50b	9.67 ± 0.57a

Control, fresh sperm.