Table 4. Results of the comet assay performed after sperm cryopreservation with 10% EG as the cryoprotective agent (CPA) and various solutions as diluents in large yellow croaker (Larimichthys crocea)
Parameters | Control | Different diluent |
ASP | Cortland | HBBS | MFRS | Stein’s |
Head length (µm) | 60.6 ± 0.65b | 56.44 ± 0.84d | 58.16 ± 0.73c | 50.70 ± 0.51e | 50.23 ± 0.74f | 61.64 ± 0.70a |
Tail length (µm) | 34.23 ± 0.52f | 39.93 ± 1.01e | 58.05 ± 1.04d | 62.11 ± 1.22c | 63.78 ± 1.37b | 120.65 ± 2.58a |
Head intensity (%) | 93.02 ± 0.69a | 87.82 ± 0.95b | 78.63 ± 0.84c | 74.31 ± 0.85d | 71.41 ± 0.86e | 57.31 ± 0.80f |
Tail intensity (%) | 6.98 ± 0.69f | 12.18 ± 0.95e | 21.37 ± 0.84d | 25.69 ± 0.85c | 28.59 ± 0.86b | 42.69 ± 0.80a |
Tail DNA (%) | 2.19 ± 0.22f | 4.19 ± 0.35e | 8.79 ± 0.39d | 10.73 ± 0.48c | 11.55 ± 0.47b | 27.95 ± 0.70a |
EG, ethylene glycol; Control, fresh sperm; ASP, artificial seminal plasma of small yellow croaker; HBBS, Hanks’ balanced salt solution; MFRS, marine fish Ringer’s solution; Stein’s, Stein’s solution.