Table 4. Results of the comet assay performed after sperm cryopreservation with 10% EG as the cryoprotective agent (CPA) and various solutions as diluents in large yellow croaker (Larimichthys crocea)

Parameters Control Different diluent
ASP Cortland HBBS MFRS Stein’s
Head length (µm) 60.6 ± 0.65b 56.44 ± 0.84d 58.16 ± 0.73c 50.70 ± 0.51e 50.23 ± 0.74f 61.64 ± 0.70a
Tail length (µm) 34.23 ± 0.52f 39.93 ± 1.01e 58.05 ± 1.04d 62.11 ± 1.22c 63.78 ± 1.37b 120.65 ± 2.58a
Head intensity (%) 93.02 ± 0.69a 87.82 ± 0.95b 78.63 ± 0.84c 74.31 ± 0.85d 71.41 ± 0.86e 57.31 ± 0.80f
Tail intensity (%) 6.98 ± 0.69f 12.18 ± 0.95e 21.37 ± 0.84d 25.69 ± 0.85c 28.59 ± 0.86b 42.69 ± 0.80a
Tail DNA (%) 2.19 ± 0.22f 4.19 ± 0.35e 8.79 ± 0.39d 10.73 ± 0.48c 11.55 ± 0.47b 27.95 ± 0.70a
EG, ethylene glycol; Control, fresh sperm; ASP, artificial seminal plasma of small yellow croaker; HBBS, Hanks’ balanced salt solution; MFRS, marine fish Ringer’s solution; Stein’s, Stein’s solution.