Table 1. Ingredient and chemical composition of the experimental diets (%, DM basis)

Variable	Experimental diets
	CP20	CP25	CP30	CP35	CP40	CP45
Ingredients (%)
Fish meal	20	24	28	32	36	40
Defatted soybean meal	10	10	10	10	10	10
Casein		2.5	5	7.5	10	12.5
Dextrin	41	34.5	28	21.5	15	8.5
Squid liver oil	0.5	0.5	0.5	0.5	0.5	0.5
Soybean oil	0.5	0.5	0.5	0.5	0.5	0.5
Sodium alginate	22	22	22	22	22	22
Mineral mix1)	4	4	4	4	4	4
Vitamin mix2)	2	2	2	2	2	2
Nutrients (%, DM basis)
Dry matter	90.5	90.3	90.3	90.4	90.4	90.5
Crude protein	20.5	25.8	31.0	35.9	41.0	46.0
Crude lipid	1.8	1.9	2.0	2.1	2.2	2.4
Ash	13.3	13.6	14.0	14.3	15.4	16.0
Carbohydrate3)	64.4	58.7	53.0	47.7	41.4	35.6
Estimated energy (kJ/g)4)	14.9	14.9	14.8	14.8	14.7	14.7

Mineral mix contained the following ingredients (g/kg mix): NaCl, 10, MgSO₄ · 7H₂O, 150; NaH₂PO₄ · 2H₂O, 250; KH₂PO₄, 320; CaH₄ (PO₄)₂ · H₂O, 200; Ferric citrate, 25; ZnSO₄ · 7H₂O, 4; Ca-lactate, 38.5; CuCl, 0.3; AlCl₃ · 6H₂O, 0.15; KIO₃, 0.03; Na₂Se₂O₃, 0.01; MnSO₄ · H₂O, 2; CoCl₂ · 6H₂O, 0.1.
Vitamin mix contained the following amount which were diluted in cellulose (g/kg mix): L-ascorbic acid, 200; α-tocopheryl acetate, 20; thiamin hydrochloride, 5; riboflavin, 8; pyridoxine, 2; niacin, 40; Ca-D-pantothenate, 12; myo-inositol, 200; D-biotin, 0.4; folic acid, 1.5; p-amino benzoic acid, 20; K₃, 4; A, 1.5; D₃, 0.003; choline chloride, 200; cyanocobalamin, 0.003.
Carbohydrate (%) = 100 – (crude protein + crude lipid + ash)%
Estimated energy (kJ/g diet) based on 16.8 kJ/g for protein and carbohydrate, and 37.8 kJ/g for lipid (Garling & Wilson, 1976).