Table 1. Base sequence information and polymerase chain reaction (PCR) conditions for the primer set used in this study

Target Primer Title 2 PCR conditions Length of seq. Reference
Prokaryote 16S rRNA Pro341F AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTCCTACGGGAGGCAGCAGCCTACGGGNBGCASCAG 95°C for 3 min, followed by 28 cycles of denaturation at 95°C for 30 sec, annealing at 55°C for 30 sec, and extension at 72°C for 30 sec, and final extension of 5 min at 72°C 550 Takahashi et al. (2014)
Pro805R CAAGCAGAAGACGGCATACGAGATNNNNNNGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTGACTACNVGGGTATCTAATCC
Vibrio splendidus 16S SpF2 ATCATGGCTCAGATTGAACG 95°C for 10 min, and followed by 40 cycles of 15 sec at 95°C and 90 sec at 60°C 150 Saulnier et al. (2017)
16S SpR2 CAATGGTTATCCCCCACATC
16S probe FAMCCCATTAACGCACCCGAAGGATTGBHQ1
Mitochondrial 16S rRNA 16Sar-5’ CGCCTGTTTATCAAAAACAT 94°C for 2 min, followed by 35 cycles of denaturation at 94°C for 30 sec, annealing at 52°C for 40 sec, and extension at 72°C for 1 min, and final extension of 10 min at 72°C 550–600 Ivanova et al. (2007);Palumbi (1996)
16Sbr-3’ CCGGTCTGAACTCAGATCACGT