Table 1. Summary of primers used in this study

Primer sequence 5'-3'	Description	Amplicon size	Tm(°C)
AGGCTGGTATCTCCAAGAA	Elongation factor 1α (LhEF1α), qPCR Forward	130 bp	60
TAACGGGACTGGCTGTAA	Elongation factor 1α (LhEF1α), qPCR Reverse	130 bp	60
GTCGCTAAACCTGACGCTTCCAAC	LhGal-1, qPCR, Forward	132 bp	60
ATTGTGTGCAACTCCTACCAGGGA	LhGal-1, qPCR, Reverse	132 bp	60
GAGAGAgatatcATGATGAAAGATCTGATCATAAAGAACATGTCCTTTAAGGT	LhGal-1, pMAL-c5X, EcoRV, Forward	408 bp	60.3
GAGAGAggatccTTACTTGATCTCAAAGCTTCTGATGCGAGC	LhGal-1, pMAL-c5X, BamHI, Reverse	408 bp	60.3

qPCR, quantitative real-time PCR; Tm, melting temperature.