Table 2. Phytotoxicity evaluation of different concentrations of AJE on zebrafish embryos

Treatment at 7 hpf
	AJE (μg/mL) in egg water
	0	5	10	50	100
Mortality (50 hpf)	0/20	0/20	0/20	0/20	0/20
Heartbeat (3 dpf)	180 ± 15	171 ± 14	186 ± 19	186 ± 19	129 ± 9*

Each group measured 12–18 embryos.
Significantly different (p < 0.05) compare with control (0 μg/mL).
hpf, hours post fertilization; AJE, Apostichopus japonicus extract; dpf, days post fertilization.