Table 2. Titers of viral hemorrhagic septicemia virus in the culture supernatant and cells at different temperatures
Temperature (°C) | Cell lines | Sampling from | Viral concentration (TCID50.· mL–1) |
Days post challenge (d) |
0 | 1 | 4 | 8 | 12 | 16 |
37 | SNU-1411 | Culture supernatant | 7.94E+04 | 2.51E+03 | 1.48E+02 | - | - | - |
Cells | - | 2.37E+01 | 3.16E+01 | 2.37E+01 | - | - |
Hepa-1c1c7s | Culture supernatant | 7.94E+04 | 3.16E+02 | - | - | - | - |
Cells | - | 4.22E+01 | 3.16E+01 | 1.88E+01 | - | - |
15 | SNU-1411 | Culture supernatant | 4.22E+06 | 3.16E+06 | 2.37E+06 | 2.37E+06 | 2.37E+05 | 6.76E+05 |
Cells | - | 3.16E+01 | 3.16E+01 | 3.16E+01 | 3.16E+01 | 3.16E+01 |
Hepa-1c1c7s | Culture supernatant | 4.22E+06 | 3.16E+06 | 1.62E+06 | 3.09E+05 | 3.09E+05 | 3.16E+05 |
Cells | – | 3.16E+01 | 3.16E+01 | 3.16E+01 | 3.16E+01 | 3.16E+01 |
SNU-1411 derived from the human tectum, hepa-1c1c7s derived from the mouse liver.
(-) indicates under detection limit (1.48E+01 TCID50.mL–1).
TCID50, 50% tissue culture infective dose.