Table 2. Biological activity of peptide from by-product fisheries and aquaculture

By product source Bioactive peptide extraction Benefits References
Skate cartilage Papain and thermal hydrolysis Improve free radical scavenging activity Li et al., 2021
Pacific white shrimp mix by-products (cephalothorax, shells, and pleopods) Trypsin autolytic hydrolysis Inhibit oxidative reactions by scavenging hydroxyl radicals Nikoo et al., 2021b
Various marine fish by-products (skin, heads, and skeletons) Collagen hydrolysis Potential antioxidant ingredients for functional foods and pharmaceuticals industry Zamorano-Apodaca et al., 2020
Abalone viscera Enzymatic hydrolysis (papain, trypsin, neutral protease, alkali protease, pepsin) Potential source of antioxidant Zhou et al., 2012
Squid pen Trypsin hydrolysis Enhance antioxidant activity Shavandi et al., 2017
Tuna dark muscle Orientase and protease XXIII hydrolysis Generate strong DPPH radical-scavenging activity and antioxidative activity Hsu, 2010
n-Hexane/ethanol extraction Inhibit the growth of obesity related diseases through the suppression of hepatic triacylglycerol and cholesterol accumulation Maeda et al., 2017
Stripped weakfish by-products (skin and bone) Alcalase and protamex hydrolysis Potential natural antimicrobial and antioxidant preservatives in food Lima et al., 2019
Bycatch shrimp Oratosquilla woodmasoni waste Thermolysin hydrolysis Produce ACE-I inhibition peptide that could be utilized as anti-hypertentsive and free radicals prevention Joshi et al., 2020
Pearl oyster shell Orientase hydrolysis Potent ACE inhibitory activity Sasaki et al., 2019
Squid skin Pepsin hydrolysis Good source of ACE inhibition peptide Lin et al., 2012
Tuna blood Enzymatic hydrolysis (alcalase, neutrase, flavourzyme) Exhibit strong antioxidant and ACE inhibitory activity Mongkonkamthorn et al., 2020
Shrimp shell Protease hydrolysis Demonstrate higher ACE inhibitory activity compared to hypertension drug (captopril) Mechri et al., 2020
Marine catfish skin Chemical extraction Possess anticancer activity against human colon cancer line Raja et al., 2020
Octopus ink Dichloromethane extraction Potential immunomodulatory and anti-proliferative against colorectal and breast cancer Hernandez-Zazueta et al., 2021
Cuttlefish posterior salivary gland toxin SDS-PAGE extraction Exhibit great cytotoxicity against breast cancer and inhibit penetration of metastatic cells Karthik et al., 2017
Rainbow trout skin Flavourzyme and alcalase hydrolysis Possess antioxidant and anticancer activities Yaghoubzadeh et al., 2020
Flathead by-products (head, backbone, skeleton) AFP hydrolysis Potent antioxidant and cancer cells cytotoxic agents Nurdiani et al., 2017
Blue mussel by-product Protamex hydrolysis Exhibit inhibitory activity against cancer cells Beaulieu et al., 2013
Sardine by-products (viscera, heads, skins, and edges) Isoelectric precipitation Improve LCAT activity and reduce complications related to obesity Affane et al., 2018
Skate skin Collagen extraction Demonstrate anti-obesity impact Woo et al., 2018
Squid by-products (viscera and ink sacs) Protease hydrolysis Suppress the activity of gram-negative and gram-positive bacteria Jiang et al., 2018
Salmon by-products (bones, fins, and tails) Protease hydrolysis Enhance copper-binding capacity Vo & Pham, 2020
Giant croaker skin Neutral protease hydrolysis Potential immunomodulatory agent Yu et al., 2020
DPPH, 2,2-diphenyl-1-picrylhydrazyl; ACE, angiotensin-converting enzyme; SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis; AFP, acid fungal protease; LCAT, leci-thin-cholesterol acyltransferase.