Table 2. Variations of osmolality, AST and ALT in plasma of olive flounder, Paralichthys olivaceus and Japanese croaker, Nibea japonica on raising water temperature sharply and continuously
Rearing days | Water temperature (°C) | Osmolality (mOsm/kg) | AST (IU/L) | ALT (IU/L) |
Olive flounder | Japanese croaker | Olive flounder | Japanese croaker | Olive flounder | Japanese croaker |
Stressed | Non-stressed | Stressed | Non-stressed | Stressed | Non-stressed | Stressed | Non-stressed | Stressed | Non-stressed | Stressed | Non-stressed | Stressed | Non-stressed |
0 | 23 | 23 | 430.5 ± 7.8bc | 430.5 ± 7.8 | 331.5 ± 5.0 | 331.5 ± 5.0a | 24.3 ± 2.1a | 24.3 ± 2.1b | 15.2 ± 12.9ab | 15.2 ± 12.9 | 23.9 ± 0.2ab | 23.9 ± 0.2c | 9.8 ± 1.9c | 9.8 ± 1.9 |
1 | 26 | 23 | 442.5 ± 3.5c | 421.0 ± 29.7 | 328.0 ± 2.8 | 326.5 ± 0.7a | 37.7 ± 4.0bc | 20.4 ± 0.1a | 17.3 ± 6.2ab | 16.6 ± 1.3 | 31.2 ± 2.5b | 21.1 ± 1.0a | 7.1 ± 1.5bc | 10.8 ± 1.1 |
5 | 23 | 24 | 447.0 ± 5.7c | NT | 333.0 ± 5.7 | NT | 37.7 ± 1.0bc | NT | 20.4 ± 0.5b | NT | 28.5 ± 5.2ab | NT | 8.6 ± 2.3c | NT |
9 | 29 | 23 | 441.5 ± 19.1c | 437.0 ± 4.2 | 331.5 ± 23.3 | 330.5 ± 2.1a | 23.9 ± 0.2a | 20.7 ± 0.5a | 23.3 ± 4.9b | 20.9 ± 0.3 | 18.3 ± 0.2a | 21.6 ± 0.2ab | 9.3 ± 2.1c | 11.6 ± 0.8 |
13 | 23 | 23 | 443.5 ± 17.7c | NT | 332.5 ± 3.5 | NT | 36.6 ± 6.4b | NT | 20.2 ± 7.5b | NT | 32.7 ± 0.4b | NT | 3.5 ± 2.3ab | NT |
17 | 32 | 22 | 435.5 ± 40.3bc | 444.5 ± 6.4 | 329.5 ± 6.4 | 334.5 ± 9.2a | 27.5 ± 2.4a | 22.2 ± 0.1ab | 2.1 ± 1.8a | 21.7 ± 1.0 | 28.2 ± 10.2ab | 22.2 ± 0.5ab | 1.4 ± 0.3a | 10.9 ± 0.7 |
21 | 23 | 23 | 397.0 ± 1.4ab | 423.5 ± 7.8 | 324.0 ± 2.6 | 331.0 ± 1.4a | 44.3 ± 2.8c | 20.6 ± 0.1a | 14.7 ± 7.6ab | 21.7 ± 1.6 | 29.0 ± 6.5ab | 21.8 ± 0.0ab | 7.7 ± 0.2c | 12.1 ± 0.4 |
60 | 17 | 17 | 386.5 ± 5.0a | 421.5 ± 17.7 | 339.5 ± 5.0 | 345.5 ± 2.1b | 26.9 ± 0.1a | 21.2 ± 0.2a | 3.0 ± 0.8a | 18.8 ± 0.2 | 21.8 ± 5.4ab | 22.5 ± 0.4b | 7.4 ± 0.2bc | 11.0 ± 4.1 |
The values are mean ± SD (olive flounder, n = 6; Japanese croaker, n = 8).
Means within each column followed by the same alphabetic letter are not significantly different (p > 0.05).
AST, aspartate aminotransferase; ALT, alanine aminotransferase; NT, not tested.