Table 1. Formulation and proximate composition of the experimental diets for olive flounder (Paralichthys olivaceus) (%, dry matter basis)

Ingredients	Experimental diets
	Thr0.4	Thr0.8	Thr1.2	Thr1.8	Thr2.0	Thr2.3
Brown fish meal	10.0	10.0	10.0	10.0	10.0	10.0
AA mix1)	40.0	40.0	40.0	40.0	40.0	40.0
Thr	0.00	0.40	0.80	1.20	1.60	2.00
Glycine	2.00	1.60	1.20	0.80	0.40	0.00
Wheat flour	10.0	10.0	10.0	10.0	10.0	10.0
Starch	20.0	20.0	20.0	20.0	20.0	20.0
Fish oil	10.0	10.0	10.0	10.0	10.0	10.0
Taurine	1.00	1.00	1.00	1.00	1.00	1.00
Mineral mix2)	2.00	2.00	2.00	2.00	2.00	2.00
Vitamin mix3)	2.00	2.00	2.00	2.00	2.00	2.00
Guar gum	2.00	2.00	2.00	2.00	2.00	2.00
Lecithin	0.50	0.50	0.50	0.50	0.50	0.50
Choline	0.50	0.50	0.50	0.50	0.50	0.50
Proximate composition (% of dry matter)
Thr	0.41	0.78	1.20	1.76	1.99	2.28
Dry matter	91.6	92.0	91.0	91.8	91.2	91.3
Crude protein	49.9	50.4	49.8	49.2	49.6	49.7
Crude lipid	10.8	10.8	10.5	10.1	10.2	10.4
Crude ash	3.21	3.77	3.35	3.84	3.31	3.87

Arginine 6.68, histidine 2.67, isoleucine 4.49, leucine 8.30, lysine 10.86, methionine 2.95, phenylalanine 4.44, tryptophan 0.67, valine 5.41, aspartic acid 10.34, glutamic acid 15.15, serine 3.78, proline 3.26, glycine 7.50, alanine 9.99, tyrosine 3.51.
MgSO₄·7H₂O, 80.0; NaH₂PO₄·2H₂O, 370.0; KCl, 130.0; ferric citrate, 40.0; ZnSO₄·7H₂O, 20.0; Ca-lactate, 356.5; CuCl, 0.2; AlCl₃. 6H₂O, 0.15; Na₂Se₂O₃, 0.01; MnSO₄·H₂O, 2.0; CoCl₂.6H₂O, 1.0.
L-ascorbic acid, 121.2; DL-a tocopheryl acetate, 18.8; thiamin hydrochloride, 2.7; riboflavin, 9.1; pyridoxine hydrochloride, 1.8; niacin, 36.4; Ca-_D-pantothenate, 12.7; myo-inositol, 181.8; _D-biotin, 0.27; folic acid, 0.68; p-aminobenzoic acid, 18.2; menadione, 1.8; retinyl acetate, 0.73; cholecalciferol, 0.003; cyanocobalamin, 0.003.
AA, amino acid; Thr, threonine.