Table 4. Non-specific immune parameters of olive flounder (Paralichthys olivaceus) fed the six experimental diets for 12 weeks

Lysozyme1) MPO2) Antiprotease3) Immunoglobulin4)
Thr0.4 8.83 ± 0.10b 0.87 ± 0.06b 19.4 ± 1.4b 10.9 ± 0.5b
Thr0.8 9.24 ± 0.09a 0.94 ± 0.07a 25.7 ± 1.4a 16.2 ± 0.9a
Thr1.2 9.27 ± 0.06a 0.93 ± 0.04a 25.1 ± 0.6a 16.4 ± 2.8a
Thr1.8 9.27 ± 0.14a 0.97 ± 0.01a 26.0 ± 0.5a 16.9 ± 1.6a
Thr2.0 9.30 ± 0.21a 0.98 ± 0.06a 25.5 ± 1.2a 16.9 ± 0.2a
Thr2.3 9.28 ± 0.08a 0.96 ± 0.02a 25.4 ± 1.5a 16.9 ± 0.6a
Pr > F*
 ANOVA 0.005 0.001 0.000 0.001
 Linear 0.002 0.000 0.000 0.000
 Quadratic 0.008 0.004 0.000 0.003
Values are mean of triplicate groups and presented as mean ± SD.
Values with different superscripts in the same column are significantly different (p < 0.05).
Lysozyme (μg/mL).
MPO activity (absorbance at 450 nm).
Antiprotease activity (% inhibition).
Total immunoglobulin (mg/mL).
Significance probability associate with the F-statistic.
MPO, myeloperoxidase; Thr, threonine; AVOVA, analysis of variance.