Table 4. Non-specific immune parameters of olive flounder (Paralichthys olivaceus) fed the six experimental diets for 12 weeks
| Lysozyme1) | MPO2) | Antiprotease3) | Immunoglobulin4) |
Thr0.4 | 8.83 ± 0.10b | 0.87 ± 0.06b | 19.4 ± 1.4b | 10.9 ± 0.5b |
Thr0.8 | 9.24 ± 0.09a | 0.94 ± 0.07a | 25.7 ± 1.4a | 16.2 ± 0.9a |
Thr1.2 | 9.27 ± 0.06a | 0.93 ± 0.04a | 25.1 ± 0.6a | 16.4 ± 2.8a |
Thr1.8 | 9.27 ± 0.14a | 0.97 ± 0.01a | 26.0 ± 0.5a | 16.9 ± 1.6a |
Thr2.0 | 9.30 ± 0.21a | 0.98 ± 0.06a | 25.5 ± 1.2a | 16.9 ± 0.2a |
Thr2.3 | 9.28 ± 0.08a | 0.96 ± 0.02a | 25.4 ± 1.5a | 16.9 ± 0.6a |
Pr > F* |
ANOVA | 0.005 | 0.001 | 0.000 | 0.001 |
Linear | 0.002 | 0.000 | 0.000 | 0.000 |
Quadratic | 0.008 | 0.004 | 0.000 | 0.003 |
Values are mean of triplicate groups and presented as mean ± SD.
Values with different superscripts in the same column are significantly different (p < 0.05).
Lysozyme (μg/mL).
MPO activity (absorbance at 450 nm).
Antiprotease activity (% inhibition).
Total immunoglobulin (mg/mL).
Significance probability associate with the F-statistic.
MPO, myeloperoxidase; Thr, threonine; AVOVA, analysis of variance.