Table 2. Effects of metallic cations on the haemolytic activities of His-tag-VpC-terminal domain and Vatlh

Concentration (mM)	Observation	Haemolysin	Relative haemolytic activity (%)
Concentration (mM)	Observation	Haemolysin	K⁺	Na⁺	Cu²⁺	Ni²⁺	Zn²⁺	Control
5.0	18	(His-tag VpTLH)	97.31 ± 3.55	98.67 ± 2.31	31.74 ± 4.61	34.85 ± 3.05	65.17 ± 11.06	100 ± 0
25.0	18	(His-tag VpTLH)	97.75 ± 2.05	94.11 ± 1.64	ND	59.34 ± 4.41	18.14 ± 9.38	100 ± 0
50.0	18	(His-tag VpTLH)	96.85 ± 3.37	93.27 ± 5.83	ND	ND	ND	100 ± 0
50.0	18	(Vatlh)	100 ± 0	100 ± 0	ND	ND	25 ± 0	100 ± 0

Metallic cations were dissolved in phosphate buffered saline.
Adapted from Jia et al. (2010) with CC-BY-NC.
Vptlh, thermolabile haemolysin of Vibrio parahaemolyticus; Vatlh, thermolabile haemolysin of Vibrio alginolyticus; ND, not determined; NA, not affect; Control, contained haemolysin without any metallic cations.