Table 8. Comparison of development time of fertilized eggs from EP or MP-fed olive flounder broodstocks

Egg developmental stage	Time lapsed post-fertilization
	EP	MP
Fertilized egg	0 h	0 h
2-cell	1 h	1 h
4-cell	1 h 30 min	1 h 40 min
8-cell	2 h	2 h 20 min
16-cell	2 h 30 min	3 h
32-cell	3 h	3 h 40 min
64-cell	3 h 30 min	4 h 20 min
128-cell	4 h	5 h
Morula	6 h	6 h 30 min
Blastula	11 h 30 min	12 h
Gastrula	15 h 30 min	16 h
Formation of embryonic body	20 h 30min	21 h 30 min
Appearance of Kupffer’s vesicle	22 h	23 h
Optic vesicle and auditory vesicle formation	26 h	28 h 30 min
Heartbeat	36 h	37 h 30 min
Before hatching (preparatory)	41 h 30 min	42 h 30 min
Hatching	43 h	44 h

EP, extruded pellet; MP, moist pellet.