Table 2. Primers used for quantitative real-time PCR

Primers¹⁾	Sequence (5`-3`)	Accession number
proPO
Forward	TCCATTCCGTCCGTCTG	AY723296.1
Reverse	GGCTTCGCTCTCGGTTAGG	AY723296.1
Lysozyme
Forward	TGTTCCGATCTGATGTCC	AY170126
Reverse	GCTGTTGTAAGCCACCC	AY170126
Crustin
Forward	GAGGGTCAAGCCTACTGCTG	AY488497
Reverse	ACTTATCGAGGCCAGCACAC	AY488497
IGF-BP
Forward	GTGGGCAGGGACCAAATC	KP420228
Reverse	TCAGTTACCACCAGCGATT	KP420228
β-actin
Forward	CGCGACCTCACAGACTACCT	AF300705
Reverse	GTGGTCATCTCCTGCTCGAA	AF300705

prophenoloxidase (proPO), insulin-like growth factor binding protein (IGF-BP).