Table 1. Sequence oligonucleotide primers used in polymerase chain reaction (PCR) amplification of GnRH genes in Pangasianodon hypophthalmus and Pangasius nasutus

Gene of interest		Primer sequence	Amplicon (bp)	Application
GnRH1	Sense	CAGGGTGACTGATCAAGGATG	~300	Full-length cloning
GnRH1	Antisense	GAAGCATTTTATTGAAGATC	~300	Full-length cloning
GnRH2	Sense	GATCTTCTCAGACCTGCACAT	~360	Full-length cloning
GnRH2	Antisense	AGGCTATGATGCTTGGGTATG	~360	Full-length cloning
GnRH3	Sense	TGGATCTTAGCAACAGAACGG	ND	Partial cloning
GnRH3	Antisense	ATGGGCACTTGTCTCCTCAG	ND	Partial cloning

GnRH, gonadotropin-releasing hormone; ND, not detected.