Table 5. Relative gene expression in the liver after 2 h of acute thermal stress at 30°C

Recovery time (h)	hsp70	irf3	gpx	cox	ampkβ	pparα	pparγ	g6pase
0	1.00 ± 0.52^a	1.00 ± 0.65^b	1.00 ± 0.15^c	1.00 ± 0.19^abc	1.00 ± 0.36^c	1.00 ± 0.14^b	1.00 ± 0.19	1.00 ± 0.35^b
2	0.07 ± 0.02^b	1.68 ± 0.55^b	1.37 ± 0.23^c	0.6 ± 0.16^bc	1.81 ± 0.55^bc	4.46 ± 0.41^a	8.56 ± 1.63	2.27 ± 0.61^ab
4	0.02 ± 0.01^b	1.81 ± 1.55^b	1.37 ± 0.18^c	0.96 ± 0.29^abc	4.33 ± 0.31^a	0.8 ± 0.11^b	14.2 ± 3.1	5.57 ± 3.35^a
6	0.00 ± 0.00^b	4.46 ± 2.49^b	1.52 ± 0.15^c	0.35 ± 0.05^c	1.65 ± 0.28^bc	1.12 ± 0.45^b	4.57 ± 0.48	5.94 ± 0.78^a
12	0.00 ± 0.00^b	3.76 ± 0.63^b	4.23 ± 0.64^b	1.34 ± 0.54ab	4.57 ± 1.42^a	1.72 ± 0.26^b	6.91 ± 1.60	2.59 ± 0.73^ab
24	0.00 ± 0.00^b	21.1 ± 15.2^a	6.88 ± 1.27^a	1.83 ± 0.33^a	4.77 ± 1.49^a	1.49 ± 0.14^b	8.82 ± 1.64	4.19 ± 1.19^a
48	0.00 ± 0.00^b	2.72 ± 1.13^b	3.13 ± 0.01^bc	1.04 ± 0.16^abc	3.77 ± 1.17^ab	1.85 ± 0.35^b	4.79 ± 0.83	3.30 ± 0.59^ab
p-value	< 0.0001	< 0.0001	0.0001	0.0038	0.0415	0.0001	0.0619	0.0140

Values are means from triplicate groups of juvenile olive flounder where the values in each row with different superscripts are significantly different (mean ± SEM; p < 0.05).
Relative gene expression level (recovery time at 0 h was set as a control; 18s used as housekeeping gene).
hsp70, heat shock protein 70; irf3, interferon regulatory factor 3; gpx, glutathione peroxidase; cox, cytochrome c oxidase; ampkβ, adenosine-activated protein kinase beta; pparα, peroxisome proliferator-activated receptors alpha; pparγ, peroxisome proliferator-activated receptors gamma; g6pase, glucose-6-phosphatase.