Table 6. Relative gene expression in the liver after 4 h of acute thermal stress at 30°C

Recovery time (h)	hsp70	irf3	gpx	cox	ampkβ	pparα	pparγ	g6pase
0	1.00 ± 0.15^a	1.00 ± 0.3^b	1.00 ± 0.19	1.00 ± 0.25^ab	1.00 ± 0.18^a	1.00 ± 0.18^a	1.00 ± 0.06^c	1.00 ± 0.25^b
2	0.18 ± 0.06^b	1.43 ± 0.37^b	0.76 ± 0.1	0.60 ± 0.14^b	0.35 ± 0.06^b	0.34 ± 0.02^bc	1.87 ± 0.26^b	2.41 ± 0.79^b
4	0.03 ± 0.01^b	16.5 ± 6.6^a	1.91 ± 0.23	0.81 ± 0.02^ab	0.37 ± 0.07^b	0.75 ± 0.13^ab	3.41 ± 0.14^a	11.3 ± 3.3^a
6	0.00 ± 0.00^b	1.69 ± 0.31^b	2.09 ± 0.32	0.46 ± 0.14^b	0.31 ± 0.03^b	0.57 ± 0.09^abc	1.10 ± 0.03^c	1.28 ± 0.28^b
12	0.00 ± 0.00^b	2.06 ± 0.71^b	2.00 ± 0.24	1.45 ± 0.32^ab	0.37 ± 0.03^b	0.43 ± 0.07^bc	0.81 ± 0.10^c	1.42 ± 0.68^b
24	0.00 ± 0.00^b	1.54 ± 0.22^b	2.54 ± 0.19	1.60 ± 0.28^a	0.37 ± 0.05^b	0.27 ± 0.05^c	0.81 ± 0.16^c	1.63 ± 0.43^b
48	0.00 ± 0.00^b	2.31 ± 1.55^b	1.36 ± 0.18	1.14 ± 0.32^ab	0.29 ± 0.05^b	0.21 ± 0.01^c	0.72 ± 0.07^c	3.23 ± 0.43^b
p-value	< 0.0001	< 0.0001	0.5543	0.0104	< 0.0001	0.0001	< 0.0001	< 0.0001

Values are means from triplicate groups of juvenile olive flounder where the values in each row with different superscripts are significantly different (mean ± SEM; p < 0.05).
Relative gene expression level (recovery time at 0 h was set as a control; 18s used as housekeeping gene).
hsp70, heat shock protein 70; irf3, interferon regulatory factor 3; gpx, glutathione peroxidase; cox, cytochrome c oxidase; ampkβ, adenosine-activated protein kinase beta; pparα, peroxisome proliferator-activated receptors alpha; pparγ, peroxisome proliferator-activated receptors gamma; g6pase, glucose-6-phosphatase.