Table 9. Relative gene expression in the brain after 4 h of acute thermal stress at 30°C

Recovery time (h)	hsp70	irf3	gpx	cox	ampkβ	pparα	pparγ	g6pase
0	1.00 ± 0.07a	1.00 ± 0.24ab	1.00 ± 0.32	1.00 ± 0.10ab	1.00 ± 0.01	1.00 ± 0.15	1.00 ± 0.19b	1.00 ± 0.54
2	0.24 ± 0.03b	1.18 ± 0.19ab	1.27 ± 0.26	0.85 ± 0.16b	0.56 ± 0.04	0.39 ± 0.05	2.61 ± 0.45a	0.05 ± 0.01
4	0.06 ± 0.02c	2.65 ± 0.57a	2.00 ± 0.30	0.97 ± 0.27ab	0.74 ± 0.12	0.81 ± 0.16	3.72 ± 0.25a	0.14 ± 0.07
6	0.01 ± 0.00c	1.19 ± 0.13ab	0.98 ± 0.05	0.83 ± 0.12b	0.6 ± 0.09	0.67 ± 0.07	1.22 ± 0.25b	0.39 ± 0.06
12	0.01 ± 0.00c	0.61 ± 0.06b	1.08 ± 0.09	1.61 ± 0.3a	0.58 ± 0.05	0.32 ± 0.06	1.14 ± 0.28b	0.2 ± 0.18
24	0.00 ± 0.00c	0.78 ± 0.05ab	0.75 ± 0.04	0.69 ± 0.1b	0.47 ± 0.07	0.61 ± 0.09	0.94 ± 0.16b	0.39 ± 0.19
48	0.00 ± 0.00c	1.15 ± 0.15ab	0.91 ± 0.05	1.01 ± 0.08ab	0.61 ± 0.05	0.64 ± 0.1	1.31 ± 0.28b	0.55 ± 0.29
p-value	< 0.0001	< 0.0001	0.1641	0.0088	0.2012	0.2665	0.0009	< 0.0001

Values are means from triplicate groups of juvenile olive flounder where the values in each row with different superscripts are significantly different (mean ± SEM; p < 0.05).
Relative gene expression level (recovery time at 0 h was set as a control; 18s used as housekeeping gene).
hsp70, heat shock protein 70; irf3, interferon regulatory factor 3; gpx, glutathione peroxidase; cox, cytochrome c oxidase; ampkβ, adenosine-activated protein kinase beta; pparα, peroxisome proliferator-activated receptors alpha; pparγ, peroxisome proliferator-activated receptors gamma; g6pase, glucose-6-phosphatase.