Table 1. Oligonucleotide primer sequence for RT-PCR

Identification	Primer name	Sequence (5’-3’)		Amplicon Size (bp)	Gene Bank
Elongation factor-1 alpha	EF1α	Forward	GAAGGAAGCTGCTGAGATGGG	776	AB122066.1
Elongation factor-1 alpha	EF1α	Reverse	GCCTCTGGGAGAGATTCGTG	776	AB122066.1
Molluscan insulin-related peptide	MIP	Forward	CAAGATCCGCTCCCTGGAAG	198	XM_011417420.2
Molluscan insulin-related peptide	MIP	Reverse	CCTCAAACTCCGCCACACTG	198	XM_011417420.2
IGF binding protein complex acid labile subunit	IGFBP_ALS	Forward	ATGTCCAAAACAATGCGTCT	369	XM_011417921.2
IGF binding protein complex acid labile subunit	IGFBP_ALS	Reverse	TCCAAAACGCGTAACTTTTC	369	XM_011417921.2
Crassostrea gigas insulin receptor-related receptor	CIR	Forward	CAAGACAGGGGAGGTGATCG	537	AJ535669.1
Crassostrea gigas insulin receptor-related receptor	CIR	Reverse	TACAGGTCCCTAGCAACCCC	537	AJ535669.1
Vitellogenin	Vg	Forward	ATGCTGGAGATGAACCTTGAG	273	AB084783.1
Vitellogenin	Vg	Reverse	TGCTTCGTGGTGGATATCCT	273	AB084783.1
Guanylyl cyclase at 76C	Gyc76C	Forward	ATGAATGCAGTGAATCGCTTGA	274	XM_011452292.2
Guanylyl cyclase at 76C	Gyc76C	Reverse	TGATACTGTGCAGCTCTTGG	274	XM_011452292.2

RT-PCR, reverse transcription-polymerase chain reaction; EF, Elongation factor; MIP, Molluscan insulin-related peptide; IGFBP_ALS, insulin-like growth factor binding protein complex acid labile subunit; CIR, Crassostrea gigas insulin receptor-related receptor.