Table 4. Relative gene expression levels of proPO and LGBP in Pacific white shrimp (Penaeus vannamei) post-larvae fed with different levels of poly-β-hydroxybutyrate (PHB) incorporated experimental diets for five weeks and challenged with toxic ammonia concentrations

	Feeding trial		Challenge test
	proPO1)	LGBP2)	proPO	LGBP
P0.0	1.00 ± 0.00c	1.00 ± 0.00	1.00 ± 0.00d	1.00 ± 0.00b
P.25	3.27 ± 0.13a	1.04 ± 0.22	0.97 ± 0.29d	1.01 ± 0.65b
P.50	1.93 ± 0.30b	1.86 ± 0.21	2.10 ± 0.18c	1.44 ± 0.28b
P1.0	3.53 ± 0.09a	1.65 ± 0.48	5.25 ± 0.28a	2.35 ± 0.04a
P2.0	1.19 ± 0.13c	1.48 ± 0.47	3.78 ± 0.86b	1.94 ± 0.28a
Pr > F*
ANOVA	0.000	0.129	0.000	0.000
Linear	0.071	0.074	0.000	0.000
Quadratic	0.000	0.148	0.550	0.107
Regression
Model	Q	NS	L	L
Adj R2	0.975	0.272	0.878	0.970

P0.0, P.25, P.50, P1.0 and P2.0 are 0.00%, 0.25%, 0.50%, 1.00% and 2.00% PHB incorporated diet treatments.
If statistical significance (p < 0.05) was detected, the model that best fit the data was chosen.
Values are the mean of triplicate (fold differences compared to the control group; n = 3) and presented as mean ± SD.
Prophenoloxidase.
Lipopolysaccharide and β-1, 3-glucan-binding protein.
Values with different superscript letters in the same column are significantly different (p < 0.05).
Significance probability associated with the F-statistic.
L, linear model; Q, quadratic model; NS, no structure; Adj R², adjusted R².