Table 1. Formulation of the basal diet for Pacific white shrimp (Penaeus vannamei)

Ingredients (g/kg)	VD0	VD45	VD90	VD135	VD270
Fish meal (sardine)1)	70	70	70	70	70
Fish meal (tuna)2)	70	70	70	70	70
Soybean meal	380	380	380	380	380
Squid liver meal	50	50	50	50	50
Wheat flour	110	110	110	110	110
Vitamin D3 premix3)	0	0.018	0.036	0.054	0.108
α-cellulose	8	7.982	7.964	7.946	7.892
Starch	200	200	200	200	200
Fish oil	40	40	40	40	40
Mineral mix4)	20	20	20	20	20
Vitamin mix (vitamin D3 free)5)	10	10	10	10	10
Cholesterol	2	2	2	2	2
Lecithin	10	10	10	10	10
Mono-calcium phosphate	30	30	30	30	30
Proximate composition (dry matter)
Crude protein	345	353	355	354	353
Crude lipid	86	86	88	86	89
Ash	96	98	97	98	98

Orizon S.A., Santiago, Chile.
Wooginfeed Industry, Incheon, Korea.
Mixture of vitamin D₃ (0.25%, DSM Nutritional Products) and cellulose to obtain graded levels of vitamin D₃. The determined dietary vitamin D₃ levels were 12, 35, 101, 132, and 294 μg/kg for VD0, VD45, VD90, VD135, and VD270, respectively.
Each kg of premix contains 12.50 g C₄H₂FeO₄, 12.00 g MnSO₄, 20.00 g FeSO₄, 6.00 g CuSO₄, 0.75 g CoSO₄, 25.00 g ZnSO₄, 0.75 g Ca(IO₃)₂, 80.20 g MgSO₄, and 0.75 g Al₂O₃. The mixture was prepared to 1 kg using cellulose.
Each kg of premix contains 1.65 g retinol, 20.0 g α-tocopherol, 5.0 g menadione, 20.0 g ascorbic acid, 2.0 g thiamin hydrochloride, 20.0 g riboflavin, 40.0 g nicotinic acid, 54.0 g myo-inositol, 18.0 g Ca-pantothenate, 15.0 g pyridoxine hydrochloride, 0.3 g biotin, 4.0 g folic acid and 0.01 g cobalamin. The mixture was prepared to 1 kg using cellulose.