Table 3. Growth performance, feed utilization and survival of Pacific white shrimp (Penaeus vannamei) fed the experimental diets for 8 weeks

Diets	IBW1)	FBW2)	WG3)	SGR4)	FCR5)	PER6)	Survival (%)
VD0	1.41 ± 0.01	5.19 ± 0.31b	7,280 ± 459b	7.41 ± 0.11b	2.03 ± 0.05a	1.43 ± 0.03b	95.0 ± 5.0
VD45	1.39 ± 0.01	5.83 ± 0.39ab	8,280 ± 501ab	7.63 ± 0.10ab	1.73 ± 0.13ab	1.65 ± 0.13ab	100.0 ± 0.0
VD90	1.41 ± 0.01	6.09 ± 0.38ab	8,562 ± 606ab	7.69 ± 0.12a	1.69 ± 0.09b	1.68 ± 0.09ab	100.0 ± 0.0
VD135	1.40 ± 0.00	6.20 ± 0.30a	8,762 ± 424a	7.73 ± 0.08a	1.60 ± 0.12b	1.78 ± 0.14a	100.0 ± 0.0
VD270	1.40 ± 0.00	5.83 ± 0.29ab	8,244 ± 414ab	7.63 ± 0.08ab	1.62 ± 0.12b	1.77 ± 0.12a	96.7 ± 2.9
Pr > F7)
ANOVA	0.109	0.032	0.031	0.027	0.016	0.054	0.109
Linear	0.489	0.030	0.029	0.024	0.002	0.008	0.496
Quadratic	0.960	0.010	0.011	0.010	0.046	0.174	0.014

Values are mean of quadruplicates (n = 4) and presented as mean ± SD. Different superscripts in each column indicate significant differences (p < 0.05). The determined dietary vitamin D₃ levels were 12, 35, 101, 132, and 294 μg/kg for VD0, VD45, VD90, VD135, and VD270, respectively.
Initial body weight (g).
Final body weight (g).
Weight gain (%) = (final body weight – initial body weight) / (initial body weight) × 100
Specific growth rate (%/day) = [(log_e final body weight – log_e initial body weight) / days] × 100
Feed conversion ratio = feed intake (g) / wet weight gain (g)
Protein efficiency ratio = wet weight gain (g) / total protein given (g)
Significance probability associate with F-statistic.
IBW, initial body weight; FBW, final body weight; WG, weight gain; SGR, specific growth rate; FCR, feed conversion ratio; PER, protein efficiency ratio; ANOVA, one-way analysis of variance.