Table 4. Hepatopancreas vitamin D₃ (μg/kg) levels and whole-body proximate composition (%, wet weight basis) of Pacific white shrimp (Penaeus vannamei) fed the experimental diets for 8 weeks

Diets	Vitamin D31)	Moisture	Crude protein	Crude lipid	Ash
VD0	2.44 ± 0.39	75.6 ± 0.7	19.9 ± 0.7	1.36 ± 0.13	3.26 ± 0.12
VD45	3.03 ± 0.92	75.5 ± 1.1	19.7 ± 0.1	1.46 ± 0.10	3.16 ± 0.16
VD90	3.69 ± 0.49	74.6 ± 1.0	20.1 ± 0.9	1.64 ± 0.25	2.98 ± 0.66
VD135	3.34 ± 0.07	74.3 ± 0.7	20.4 ± 0.8	1.50 ± 0.17	3.07 ± 0.47
VD270	3.79 ± 0.52	75.4 ± 1.5	20.2 ± 0.2	1.43 ± 0.17	2.87 ± 0.46
Pr > F2)
ANOVA	0.230	0.300	0.745	0.272	0.740
Linear	0.057	0.360	0.280	0.534	0.220
Quadratic	0.412	0.133	0.956	0.060	0.930

Values are mean of quadruplicate groups and presented as mean ± SD. Values with different superscripts in the same column indicate significantly different (p < 0.05). The determined dietary vitamin D₃ levels were 12, 35, 101, 132, and 294 μg/kg for VD0, VD45, VD90, VD135, and VD270, respectively. The lack of superscript letter indicates no significant difference among the treatments.
Vitamin D₃ level (mg/kg).
Significance probability associate with F-statistic.
ANOVA, one-way analysis of variance.