Table 6. Non-specific immunity in hemolymph of Pacific white shrimp (Penaeus vannamei) fed the experimental diets for 8 weeks

Diets	PO1)	Lysozyme2)	NBT3)
VD0	0.27 ± 0.06	13.06 ± 1.43bc	1.40 ± 0.32bc
VD45	0.31 ± 0.03	11.26 ± 0.78c	1.18 ± 0.10c
VD90	0.38 ± 0.11	13.50 ± 0.90ab	1.74 ± 0.34bc
VD135	0.29 ± 0.06	13.96 ± 0.62ab	2.07 ± 0.40b
VD270	0.27 ± 0.05	15.24 ± 1.31a	2.90 ± 0.69a
Pr > F4)
ANOVA	0.077	0.000	0.000
Linear	0.852	0.000	0.000
Quadratic	0.019	0.024	0.007

Values are mean of quadruplicate (n = 4) and presented as mean ± SD. Different superscripts in each column indicate significant differences (p < 0.05). The determined dietary vitamin D₃ levels were 12, 35, 101, 132, and 294 μg/kg for VD0, VD45, VD90, VD135, and VD270, respectively.
Phenoloxidase activity (absorbance).
Lysozyme activity (μg/mL).
Nitro-blue tetrazolium activity (absorbance).
Significance probability associate with F-statistic.
PO, phenoloxidase; NBT, nitro-blue tetrazolium; ANOVA, one-way analysis of variance.