Table 8. Hepatopancreas histomorphology of Pacific white shrimp (Penaeus vannamei) fed the experimental diets for 8 weeks

Diets B cell counts1) B cell diameter2) LD3)
VD0 3.73 ± 0.45d 27.4 ± 1.10d 19.1 ± 1.41c
VD45 4.43 ± 0.50c 28.0 ± 1.80d 52.3 ± 3.27a
VD90 5.57 ± 0.50b 35.1 ± 2.00b 41.9 ± 5.94b
VD135 5.80 ± 0.41b 32.0 ± 1.21c 42.1 ± 3.10b
VD270 7.50 ± 1.01a 39.2 ± 2.86a 21.4 ± 2.34c
Pr > F4)
 ANOVA 0.000 0.000 0.000
 Linear 0.000 0.000 0.005
 Quadratic 0.010 0.025 0.000
Values are mean of quadruplicate (n = 4) and presented as mean ± SD. Different superscripts in each column indicate significant differences (p < 0.05). The determined dietary vitamin D3 levels were 12, 35, 101, 132, and 294 μg/kg for VD0, VD45, VD90, VD135, and VD270, respectively.
B cell counts (number/tubule).
B cell diameter (μm).
Lipid droplet (number/tubule).
Significance probability associate with F-statistic.
LD, lipid droplet; ANOVA, one-way analysis of variance.