Table 1. Primers used for cDNA cloning and expression analysis of PvGrx5

Primer name	Sequence (5^'–3^’)	Purpose
3^' RACE-1	AGTGCACAAGTCGGAAGTAAA	RACE PCR
3^' RACE-2	GACAGTGTTCGACAAGGGATTA
5^' RACE-1	CTGAGGTGATGCCAACTTTCT
5^' RACE-2	CTTGTGGAATTGTTGGCCAGTCTG
PvGrx5 qPCR-F	GTTGGCATCACCTCAGCCTTATT	RT-qPCR
PvGrx5 qPCR-R	AAGCCTTGTACCTGAACCTATGG
Pv EF1α qPCR-F	TCGCCGAACTGCTGACCAAGA
Pv EF1α qPCR-R	CCGGCTTCCAGTTCCTTACC
ORF (TA-clone)-F	CCAGGCGTAAACACTTTCCT	ORF cloning
ORF (TA-clone)-R	CAAGCCTTGTACCTGAACCTATG	ORF cloning
PvGrx5-Exp-F¹⁾	cacacaAAGCTTTGCAGTGCACAAGTCGGA	Recombinant protein expression
PvGrx5-Exp-R¹⁾	ctctctGAATTCTCATTTCTTTTCTCCTTT	Recombinant protein expression

Lowercase letters indicate the protective bases, and the sequences AAGCTT and GAATTC correspond to the restriction sites for HindIII and EcoRI, respectively.
PvGrx5, Penaeus vannamei glutaredoxin 5; RACE, rapid amplification of cDNA ends; PCR, polymerase chain reaction; RT-qPCR, reverse Transcription-quantitative polymerase chain reaction; ORF, open reading frame; TA, Taq DNA polymerase.