Table 2. Primer sequences of target genes used in real-time quantitative PCR

Target gene	Primer sequences (5’ to 3’)	Accession number/reference
β-Actin	(F): 5’-GAGCAACACGGAGTTCGTTGT-3’(R): 5’-CATCACCAACTGGGACGACATGGA-3’	AF300705.2
EF1-α	(F): 5’-GAAGTAGCCGCCCTGGTTG-3’(R): 5’-CGGTTAGCCTTGGGGTTGAG-3’	XM027373349.1
proPO	(F): 5’-TTCAACGGTAGACCCGTGATTCTTC-3’(R): 5’-TCTTGCCGGGTTTAAGGTGAACAGT-3’	AY723296.1
Crustin	(F): 5’-CTTGCACACGTGTTCTCCCAAACA-3’(R): 5’-ACCAAGATACTCGACTGCCCACAA-3’	AY486426.1
IGF-BP	(F): 5’-GTGGGCAGGGACCAAATC-3’(R): 5’-TCAGTTACCACCAGCGATT-3’	Lee et al. (2021)
IKBK	(F): 5’-GCAAGAACCGCACAACACAT-3’(R): 5’-AAGCATTAGGAACGGGCTGG-3’	JN180642.1
TGF-β	(F): 5’-CGCATACACCTATACGAGAG-3’(R): 5’-CTGGTGCTGAGTCATATCTG-3’	MH259763.1
TLR3	(F): 5’-TGACGACCTGCATGAACACCTCTT-3’(R): 5’-GGTGCCACGGTACACGAAACATAAC-3’	JN180638.1

PCR, polymerase chain reaction; EF1-α, elongation factor 1α; proPO, prophenoloxidase; IGF-BP, insulin-like growth factor binding protein; IKBK, inhibitor of nuclear factor kappa-β kinase subunit beta; TGF-β, transforming growth factor beta; TLR3, toll-like receptor 3.